chicken ova323 339 peptide Search Results


ova 257 264 peptide  (InvivoGen)
96
InvivoGen ova 257 264 peptide
Identification of astemizole and ikarugamycin as immunostimulatory agents. (A–D) De-ini dendritic cells (DC) were cultured with the compounds of the indicated chemical libraries for 16 hours, then washed twice and pulsed with 500 µg/mL ovalbumin <t>(OVA)</t> protein for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells for 16 hours. Then, the supernatant of cells was harvested and the production of interleukin 2 (IL2), as an indicator for antigen cross-presentation capacity, was measured by ELISA. IL2 concentration was normalized according to dimethyl sulfoxide (DMSO)-treated controls. (E–F) De-iniDCs and bone marrow-derived DCs (BMDCs) were treated with DMSO or ikarugamycin (0.1, 0.2, 0.5, 1 µM) (E), or astemizole (0.2, 0.5, 1, 2, 5 µM) (F) for 16 hours, then washed twice and pulsed with OVA protein or 10 ng/mL OVA257-264 peptide <t>(SL8,</t> as a positive control) for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells. IL-2 secretion was measured by ELISA as an indication for antigen cross-presentation capacity. Data are reported as the mean±SD of three replicates, and statistical analyses were performed with a two-tailed unpaired Student’s t test. de-iniDCs, de-induced/de-immortalized DCs.
Ova 257 264 Peptide, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chicken ova323 339 peptide  (Biosynth Carbosynth)
92
Biosynth Carbosynth chicken ova323 339 peptide
Identification of astemizole and ikarugamycin as immunostimulatory agents. (A–D) De-ini dendritic cells (DC) were cultured with the compounds of the indicated chemical libraries for 16 hours, then washed twice and pulsed with 500 µg/mL ovalbumin <t>(OVA)</t> protein for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells for 16 hours. Then, the supernatant of cells was harvested and the production of interleukin 2 (IL2), as an indicator for antigen cross-presentation capacity, was measured by ELISA. IL2 concentration was normalized according to dimethyl sulfoxide (DMSO)-treated controls. (E–F) De-iniDCs and bone marrow-derived DCs (BMDCs) were treated with DMSO or ikarugamycin (0.1, 0.2, 0.5, 1 µM) (E), or astemizole (0.2, 0.5, 1, 2, 5 µM) (F) for 16 hours, then washed twice and pulsed with OVA protein or 10 ng/mL OVA257-264 peptide <t>(SL8,</t> as a positive control) for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells. IL-2 secretion was measured by ELISA as an indication for antigen cross-presentation capacity. Data are reported as the mean±SD of three replicates, and statistical analyses were performed with a two-tailed unpaired Student’s t test. de-iniDCs, de-induced/de-immortalized DCs.
Chicken Ova323 339 Peptide, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse mog 35–55 peptide  (AnaSpec)
90
AnaSpec mouse mog 35–55 peptide
Identification of astemizole and ikarugamycin as immunostimulatory agents. (A–D) De-ini dendritic cells (DC) were cultured with the compounds of the indicated chemical libraries for 16 hours, then washed twice and pulsed with 500 µg/mL ovalbumin <t>(OVA)</t> protein for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells for 16 hours. Then, the supernatant of cells was harvested and the production of interleukin 2 (IL2), as an indicator for antigen cross-presentation capacity, was measured by ELISA. IL2 concentration was normalized according to dimethyl sulfoxide (DMSO)-treated controls. (E–F) De-iniDCs and bone marrow-derived DCs (BMDCs) were treated with DMSO or ikarugamycin (0.1, 0.2, 0.5, 1 µM) (E), or astemizole (0.2, 0.5, 1, 2, 5 µM) (F) for 16 hours, then washed twice and pulsed with OVA protein or 10 ng/mL OVA257-264 peptide <t>(SL8,</t> as a positive control) for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells. IL-2 secretion was measured by ELISA as an indication for antigen cross-presentation capacity. Data are reported as the mean±SD of three replicates, and statistical analyses were performed with a two-tailed unpaired Student’s t test. de-iniDCs, de-induced/de-immortalized DCs.
Mouse Mog 35–55 Peptide, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antigenic peptide ova 323–339  (GenScript corporation)
90
GenScript corporation antigenic peptide ova 323–339
Identification of astemizole and ikarugamycin as immunostimulatory agents. (A–D) De-ini dendritic cells (DC) were cultured with the compounds of the indicated chemical libraries for 16 hours, then washed twice and pulsed with 500 µg/mL ovalbumin <t>(OVA)</t> protein for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells for 16 hours. Then, the supernatant of cells was harvested and the production of interleukin 2 (IL2), as an indicator for antigen cross-presentation capacity, was measured by ELISA. IL2 concentration was normalized according to dimethyl sulfoxide (DMSO)-treated controls. (E–F) De-iniDCs and bone marrow-derived DCs (BMDCs) were treated with DMSO or ikarugamycin (0.1, 0.2, 0.5, 1 µM) (E), or astemizole (0.2, 0.5, 1, 2, 5 µM) (F) for 16 hours, then washed twice and pulsed with OVA protein or 10 ng/mL OVA257-264 peptide <t>(SL8,</t> as a positive control) for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells. IL-2 secretion was measured by ELISA as an indication for antigen cross-presentation capacity. Data are reported as the mean±SD of three replicates, and statistical analyses were performed with a two-tailed unpaired Student’s t test. de-iniDCs, de-induced/de-immortalized DCs.
Antigenic Peptide Ova 323–339, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chicken ova323–339 (isqavhaahaeineagr)  (Peptron Inc)
90
Peptron Inc chicken ova323–339 (isqavhaahaeineagr)
Identification of astemizole and ikarugamycin as immunostimulatory agents. (A–D) De-ini dendritic cells (DC) were cultured with the compounds of the indicated chemical libraries for 16 hours, then washed twice and pulsed with 500 µg/mL ovalbumin <t>(OVA)</t> protein for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells for 16 hours. Then, the supernatant of cells was harvested and the production of interleukin 2 (IL2), as an indicator for antigen cross-presentation capacity, was measured by ELISA. IL2 concentration was normalized according to dimethyl sulfoxide (DMSO)-treated controls. (E–F) De-iniDCs and bone marrow-derived DCs (BMDCs) were treated with DMSO or ikarugamycin (0.1, 0.2, 0.5, 1 µM) (E), or astemizole (0.2, 0.5, 1, 2, 5 µM) (F) for 16 hours, then washed twice and pulsed with OVA protein or 10 ng/mL OVA257-264 peptide <t>(SL8,</t> as a positive control) for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells. IL-2 secretion was measured by ELISA as an indication for antigen cross-presentation capacity. Data are reported as the mean±SD of three replicates, and statistical analyses were performed with a two-tailed unpaired Student’s t test. de-iniDCs, de-induced/de-immortalized DCs.
Chicken Ova323–339 (Isqavhaahaeineagr), supplied by Peptron Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chicken ova peptide ova 323–339  (Neo MPS Inc)
90
Neo MPS Inc chicken ova peptide ova 323–339
Identification of astemizole and ikarugamycin as immunostimulatory agents. (A–D) De-ini dendritic cells (DC) were cultured with the compounds of the indicated chemical libraries for 16 hours, then washed twice and pulsed with 500 µg/mL ovalbumin <t>(OVA)</t> protein for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells for 16 hours. Then, the supernatant of cells was harvested and the production of interleukin 2 (IL2), as an indicator for antigen cross-presentation capacity, was measured by ELISA. IL2 concentration was normalized according to dimethyl sulfoxide (DMSO)-treated controls. (E–F) De-iniDCs and bone marrow-derived DCs (BMDCs) were treated with DMSO or ikarugamycin (0.1, 0.2, 0.5, 1 µM) (E), or astemizole (0.2, 0.5, 1, 2, 5 µM) (F) for 16 hours, then washed twice and pulsed with OVA protein or 10 ng/mL OVA257-264 peptide <t>(SL8,</t> as a positive control) for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells. IL-2 secretion was measured by ELISA as an indication for antigen cross-presentation capacity. Data are reported as the mean±SD of three replicates, and statistical analyses were performed with a two-tailed unpaired Student’s t test. de-iniDCs, de-induced/de-immortalized DCs.
Chicken Ova Peptide Ova 323–339, supplied by Neo MPS Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ova 323 339 isqavhaahaeineagr  (Biosynth Carbosynth)
94
Biosynth Carbosynth ova 323 339 isqavhaahaeineagr
Identification of astemizole and ikarugamycin as immunostimulatory agents. (A–D) De-ini dendritic cells (DC) were cultured with the compounds of the indicated chemical libraries for 16 hours, then washed twice and pulsed with 500 µg/mL ovalbumin <t>(OVA)</t> protein for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells for 16 hours. Then, the supernatant of cells was harvested and the production of interleukin 2 (IL2), as an indicator for antigen cross-presentation capacity, was measured by ELISA. IL2 concentration was normalized according to dimethyl sulfoxide (DMSO)-treated controls. (E–F) De-iniDCs and bone marrow-derived DCs (BMDCs) were treated with DMSO or ikarugamycin (0.1, 0.2, 0.5, 1 µM) (E), or astemizole (0.2, 0.5, 1, 2, 5 µM) (F) for 16 hours, then washed twice and pulsed with OVA protein or 10 ng/mL OVA257-264 peptide <t>(SL8,</t> as a positive control) for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells. IL-2 secretion was measured by ELISA as an indication for antigen cross-presentation capacity. Data are reported as the mean±SD of three replicates, and statistical analyses were performed with a two-tailed unpaired Student’s t test. de-iniDCs, de-induced/de-immortalized DCs.
Ova 323 339 Isqavhaahaeineagr, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chicken ova (323-339)  (LifeTein Inc)
90
LifeTein Inc chicken ova (323-339)
KEY RESOURCES TABLE
Chicken Ova (323 339), supplied by LifeTein Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ot-ii transgenic mice  (Jackson Laboratory)
90
Jackson Laboratory ot-ii transgenic mice
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Ot Ii Transgenic Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chicken ova 323–339 peptide  (GenScript corporation)
90
GenScript corporation chicken ova 323–339 peptide
KEY RESOURCES TABLE
Chicken Ova 323–339 Peptide, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-chicken ova(323-339) igg  (Alpha Diagnostics)
90
Alpha Diagnostics rabbit anti-chicken ova(323-339) igg
KEY RESOURCES TABLE
Rabbit Anti Chicken Ova(323 339) Igg, supplied by Alpha Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chicken ova323-339 peptide  (Millipore)
90
Millipore chicken ova323-339 peptide
KEY RESOURCES TABLE
Chicken Ova323 339 Peptide, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Identification of astemizole and ikarugamycin as immunostimulatory agents. (A–D) De-ini dendritic cells (DC) were cultured with the compounds of the indicated chemical libraries for 16 hours, then washed twice and pulsed with 500 µg/mL ovalbumin (OVA) protein for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells for 16 hours. Then, the supernatant of cells was harvested and the production of interleukin 2 (IL2), as an indicator for antigen cross-presentation capacity, was measured by ELISA. IL2 concentration was normalized according to dimethyl sulfoxide (DMSO)-treated controls. (E–F) De-iniDCs and bone marrow-derived DCs (BMDCs) were treated with DMSO or ikarugamycin (0.1, 0.2, 0.5, 1 µM) (E), or astemizole (0.2, 0.5, 1, 2, 5 µM) (F) for 16 hours, then washed twice and pulsed with OVA protein or 10 ng/mL OVA257-264 peptide (SL8, as a positive control) for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells. IL-2 secretion was measured by ELISA as an indication for antigen cross-presentation capacity. Data are reported as the mean±SD of three replicates, and statistical analyses were performed with a two-tailed unpaired Student’s t test. de-iniDCs, de-induced/de-immortalized DCs.

Journal: Journal for Immunotherapy of Cancer

Article Title: Anticancer effects of ikarugamycin and astemizole identified in a screen for stimulators of cellular immune responses

doi: 10.1136/jitc-2023-006785

Figure Lengend Snippet: Identification of astemizole and ikarugamycin as immunostimulatory agents. (A–D) De-ini dendritic cells (DC) were cultured with the compounds of the indicated chemical libraries for 16 hours, then washed twice and pulsed with 500 µg/mL ovalbumin (OVA) protein for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells for 16 hours. Then, the supernatant of cells was harvested and the production of interleukin 2 (IL2), as an indicator for antigen cross-presentation capacity, was measured by ELISA. IL2 concentration was normalized according to dimethyl sulfoxide (DMSO)-treated controls. (E–F) De-iniDCs and bone marrow-derived DCs (BMDCs) were treated with DMSO or ikarugamycin (0.1, 0.2, 0.5, 1 µM) (E), or astemizole (0.2, 0.5, 1, 2, 5 µM) (F) for 16 hours, then washed twice and pulsed with OVA protein or 10 ng/mL OVA257-264 peptide (SL8, as a positive control) for 4 hours, washed again twice, and then co-cultured with B3Z hybridoma cells. IL-2 secretion was measured by ELISA as an indication for antigen cross-presentation capacity. Data are reported as the mean±SD of three replicates, and statistical analyses were performed with a two-tailed unpaired Student’s t test. de-iniDCs, de-induced/de-immortalized DCs.

Article Snippet: OVA 257–264 peptide (SL8, Cat# vac-sin) was obtained from Invivogen (San Diego, California, USA).

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Concentration Assay, Derivative Assay, Positive Control, Two Tailed Test

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation

doi: 10.1016/j.celrep.2020.107820

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Chicken OVA (323-339) , Lifetein , Cat# LT51023.

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Sonication, DNA Library Preparation, Purification, Activation Assay, cDNA Synthesis, Software